Journal: bioRxiv
Article Title: Profilin 1 maintains cell cycle fidelity to prevent unscheduled genome doubling and polyploidy in cancer
doi: 10.64898/2026.05.12.724607
Figure Lengend Snippet: Profilin 1 depletion increases the metastatic potential of osteosarcoma cells a. Schematic overview of the intratibial injection of SaOS2 cells into the bone marrow cavity of NOD-SCID-Gamma (NSG) mice and subsequent tissue collection at endpoint. b. Representative photographs of injected and contralateral non-injected hind limbs of NSG mice showing osteosarcoma growth at the joint region. c. Table summarizing osteosarcoma mass size and the number of metastatic nodules detected in the lungs and liver of NSG mice injected with parental (PAR) or PFN1 KO SaOS2 cells. d. Representative hematoxylin and eosin (H&E)–stained lung section showing normal alveolar architecture and infiltration by metastatic osteosarcoma cells (blue contour). Bottom, higher-magnification view of the metastatic lesion. e. Quantification of the number of metastatic sites detected across lung sections from NSG mice injected with parental or PFN1 KO SaOS2 cells.
Article Snippet: Six-week-old male NOD scid gamma (NSG) mice were purchased from Charles River Laboratories and housed under controlled environmental conditions with ad libitum access to food and water.
Techniques: Injection, Staining